Detailed Quote Request

Please complete this form as thoroughly as possible to facilitate your initial conversation with a Blue Sky representative.

Contact Information
Name:
Organization:
Address:
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City:
State:
Zip/Postal Code:
Country:
Email:
Phone Number:
Preferred Contact:	email phone
Nature of inquiry:	Pricing estimate Quoting

General Information
Service(s) desired:	CloningExpressionPurification	Other:
Application for Final Deliverable:	Immunogen Screening Structural Biotherapeutic Other	Other:

SECTION I - Target Information
Protein Name:
Species:
MW:
Accession #:
Target Amino Acid Sequence:
Affinity Tag(s):	N-Term: His Puritin™ RedSee™ FLAG Sumo MBP HA MYC Strep-II Other   C-Term: His Puritin™ RedSee™ FLAG Sumo MBP HA MYC Strep-II Other   Other:   Other:
Comments:

SECTION II - Molecular Biology
	Gene SynthesisPCR Subcloning from Existing Vectors   Gene Synthesis     PCR Subcloning from Existing Vector Codon Optimization: E. coli Yeast Insect Mammalian Starting Material: Commercial Client Supplied Vector Name: Attach Vector Map Upload:Upload file Other:
Deliverable:	Purified PlasmidGlycerol StockLarge Scale Plasmid PrepAdvance to expression
Comments:

SECTION III - Expression Service
Expression System:	E. coli Yeast Baculovirus Drosophila S2 Mammalian Other:	Other::
Starting Materials:	Plasmid Expression strain or virus stock
Expression Optimization:	Multiple strains/cell linesMultiple time points
Pilot Expression:	36 ml 50 ml 125 ml 250 ml 500 ml
Expression Scale:	1L 5L 10L 20L 50L 100L To be determined Other	Other:
Target Detection:	SDS-PAGE Anti-Tag Immunoblot Anti-Target Immunoblot Other	Other:
Deliverable:	Cell PasteConditioned MediumAdvance to Purification
Comments:

SECTION IV - Purification Service
Purification Method:	Method development required
	One-step Affinity Chromatography   Tag removal required?: Yes No N/A
	Resin Screening (e.g. ion exchange, hydrophobic interaction, etc.)
	Based on published method (Please provide reference (PDF file preferred)) Upload:Upload file
Product Requirements:	Desired Purity: Yield needed:   Concentration:
Final Formulation:	Storage Buffer Composition:    Dispensing Size:    Storage Temp:
Special Requirements:	Activity:    Endotoxin Limit:    Other:
Biophysical Requirements:	Mass Spec Immunoblot Other   Other:
Comments:

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All reagents produced are for research purposes only.