Blue Sky Biotech is a premium CRO for research-based drug discovery. The Company currently holds contracts for its services with the majority of Fortune’s Top Ten Global Pharmaceutical & Biotech Companies as well as leading academic and non-profit institutes. Blue Sky sells MORE TIME to its clients, enabling pursuit of innovative medicines for unmet therapeutic needs.
Click on a service category at the left to explore all of our service offerings. Contact us to learn more about how Blue Sky can enable your research.
Molecular Biology
- Gene (cDNA) cloning
- Gene subcloning
- Vector construction and modification
- Site-directed mutagenesis
- Random mutagenesis
- Construct design
- Parallel generation of multiple constructs
- Molecular genetics: knockouts, RNAi, Northern blots, and Southern blots
- QC supported by DNA sequencing service
- Sequence assembly
Cell Culture and Scale-Up (Insect and Mammalian Cell Lines)
- Expression in baculovirus system: baculovirus generation and amplification, infection of insect cells with recombinant baculovirus.
- Expression optimization in baculovirus: cell line, temperature, MOI, VOI, growth conditions, media
- Expression in mammalian cells
- Expression optimization in mammalian cells: cell line, transfection conditions, growth conditions, media
- Viral production (insect and mammalian)
Cell Culture and Scale-Up (Bacterial and Yeast Fermentation)
- Expression in common E. coli systems: T7 promoter (pET) system, traditional lac and tac systems, other systems (trp, tet, etc.)
- Expression in yeast systems
- Expression optimization in E. coli: varied strain, temperature, inducer, growth conditions, media, construct
- Scale-up of E. coli in multiple 10 L bioreactors
- Scale-up of P. pastoris and other yeasts in multiple 10 L bioreactors
- Optimization of fermentation conditions (including multiple parallel trials to determine best conditions)
- Process development for fermentation
- Perfusion and microcarrier culture
- Batch, fed-batch and continuous culture
- Intracellular and secreted products
Protein Purification
- Tissue/organ extraction and purification
- Ion exchange chromatography
- Size exclusion chromatography
- Affinity chromatography: tag columns (His, etc.) and non-tag affinity (blue, etc.)
- Development of multi-step protocols
- Activity validation available (when applicable) at each step of the process
- QC supported by Mass Spec service
- QC by Western blot and SDS-PAGE
- Protein Analysis by Agilent Bioanalyzer
- Endotoxin testing by Endosafe-PTS and endotoxin removal
Assay Design
- Apoptosis Assay development and execution
- Custom Lipid Vesicle Reagent Construction
- ELISA development and optimization
- Enzyme and enzyme-linked in vitro assays (end-point and kinetic, multiple wavelengths) FRET, TR-FRET, BRET
- IC50 Determinations in both in vitro enzyme and cell-based assay systems
- Luciferase Reporter Gene Assays
- MIC (Minimum Inhibitory Concentration) antibiotic assays