About one third of the proteins encoded by the human genome are membrane proteins; these represent more than half of potential drug targets. The biological context, structure and organization provided by a membrane is often ignored in typical biochemical assays. Our patented reagent, TDA 2.0™, easily facilitates restoration of the membrane context and is featured in our SmartScreen™ kits.
An assay design and development service
TDA 2.0™ is composed of a stable, soluble vesicle made from a chemically modified lipid which is capable of binding polyhistidine-tagged proteins. Since it is a vesicle comprised of a lipid bilayer, it serves to provide a surface similar to a biological membrane. The membrane is likely to be a critical component when examining membrane proteins as it provides a context which orients the enzyme (relative to the membrane and to other subunits), dictates topology of the enzyme, and modifies the effective concentration of the enzyme.
Presentation of enzymes which are naturally membrane-associated using TDA 2.0™ allows the enzymes to behave in a more biologically relevant way.
We offer collaborative kit development through two paths:
- Enzymes already in our development pipeline can be expedited through partnership.
- TDA 2.0™-optimized reagents can be rapidly custom-produced using a milestone-based fee-for-service contract.
If you don't see your target on our site, tell us! Our team of scientists will collaboratively complete assay development services, including custom protein production and field application service in your laboratory, for maximum flexibility.
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Custom Assay Development
While we have dozens of kits available or in development, we recognize that drug discovery won't wait. TDA my way provides the flexibility of custom kits and assay development services to expedite the optimization of your target in the TDA platform.
Design of a TDA-enabled assay includes some or all of the following aspects:
- Design optimal gene construct that will be compatible with the TDA technology
- Express and purify your protein
- Determine initial assay conditions
- Interrogate a library of substrates to find the optimal candidate(s)
- Optimize the assay to support the format of your choice (HTRF, Caliper, etc...)
- Transfer the assay protocol to your laboratory including on-site support
- Run inhibitor profiles and or compound screens